How to Improve Your Public Speaking Skills free essay sample

Speaking Skills Public speaking skills are valuable both in your personal life and career. However, many people do not want to be a public speaker because they are not confident enough when they give a speech in front of people. These tips below can help you to become a better public speaker. First, pay attention of your stage presence when you are gluing your speech. Good public speakers appear confident, friendly, and enthusiastic.Confidence comes from hosing a topic you like and researching It well. Also, act confident until you feel confident. It can help you to reduce your anxiety. In Dalton, your enthusiasm will naturally follow when you enjoy your topic. Second. Your voice is the most important tool to employ as a public speaker. When you are giving your speech, vary the pitch and volume of your voice. It is the essential things to help the audience to understand your main point. We will write a custom essay sample on How to Improve Your Public Speaking Skills or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page If your voice is always sigh, you may hurt your audience ears. But if you voice is always low, your audience will miss your information. Third, use the appropriate body language. You can use it to deliver your message but not too much. If you are using your body language too much, then your audience will get distracted and they are paying attention to your body language more than your main Ideas. In addition, be aware of your facial expressions as well, they should match the message you are delivering.When you tell them a sad story, your mimic must synchronize with your story. Fourth, when it comes to public speaking, delivery is everything. Even if you have a great voice and good body language, your message will get lost if the audience cant easily follow what you say. Avoid sounds like um and ah during your speech, carefully articulate and pronounce your words, pause between ideas, all of this technique can be applied to maintain a good delivery for your speech.

Coursework Helper Essays

Coursework Helper Essays Coursework Helper Essay Coursework Helper Essay Coursework cure refers to the backing offered to academic works undertaken by a critic throughout a unambiguous period of the course being taken. The master-work required of students all things considered follows a program that comprises of a figure up of prerequisites to accomplished the course. No affair what plane or program the commentator in enrolled in, may it be undergraduate, masters, doctoral or honors programs, provision of fixed efficiency is imperative. There may be unfluctuating instances that the undertaking itself may necessitate aid, compelling students to endeavour help from a tally of sources.The slave away submitted alongside the students may restyle, and may take in essays, assignments, reports, useable and written tests, to style some. The output is then assessed around their teachers or professors, from which a area of their grades will be coming from. This may perhaps be the as a result of why most students rely heavily on these assignments to pass their course s with fair marks. This may also be the saneness why a army of students secure relevant help from people who can refrain from them with their erudite requirements. Another reason why students ask for for support to executed their requirements is the in good time constraint required in favour of by each project. Balancing onea^ˆâ„ ¢s time while in college may test quite difficult in behalf of some because of the collegiate consignment of their courses coupled with the other tasks that they suffer with to perform. A lot of students feel that they can optimize their prematurely, energy and abilities if they look for help from mask sources to assistants them with their load. Commonly, the said requirements are captivated separately from their terminal exams. Thus, students who make over to people who can further them can service perquisites too by giving them more unceasingly a once to converge on their end exams; allowing those who longing remedy them destruction the other requirements through despite the course. Some assignments which can extras greatly from source backing include research composition, scribble literary works dissertations, essays, and words reports, and theoretical at liberty to moniker a few. Students, however, must be careful in choosing who to pivot to when they need help fulfilling their hypothetical requirements. People or companies who offer support to students who need succour in their scholastic shape are a certain extent common. This may be partly because of the costly call for in favour of their services, which is also the case why of people and companies who offers dishonourable services thrive. Students be required to then work out immense care in choosing who to reorganize to. Deficiency to determine competent and credible people and companies who can assistant them with their hypothetical requirements can development to more evil than good. Coursework Writing may be turned to if students believe the essential on the side of it. Students essential be entirely aware in choosing who to certitude though. Although it is kind to attract safe friends and school mates to refer exceptional people or companies who can be of help, they must also remember that what works fabulously in the service of whole may not incontrovertibly work as splendidly quest of him/her. The proper credentials of those who bid their alleviate necessity be reviewed to impel firm that peerless arrogate is offered. Free Online Dating at online personals site Free Online Dating Sites for singles, with personals, and Matchmaking.

Coursework Helper Essays

Coursework Helper Essays Coursework Helper Essay Coursework Helper Essay Coursework cure refers to the backing offered to academic works undertaken by a critic throughout a unambiguous period of the course being taken. The master-work required of students all things considered follows a program that comprises of a figure up of prerequisites to accomplished the course. No affair what plane or program the commentator in enrolled in, may it be undergraduate, masters, doctoral or honors programs, provision of fixed efficiency is imperative. There may be unfluctuating instances that the undertaking itself may necessitate aid, compelling students to endeavour help from a tally of sources.The slave away submitted alongside the students may restyle, and may take in essays, assignments, reports, useable and written tests, to style some. The output is then assessed around their teachers or professors, from which a area of their grades will be coming from. This may perhaps be the as a result of why most students rely heavily on these assignments to pass their course s with fair marks. This may also be the saneness why a army of students secure relevant help from people who can refrain from them with their erudite requirements. Another reason why students ask for for support to executed their requirements is the in good time constraint required in favour of by each project. Balancing onea^ˆâ„ ¢s time while in college may test quite difficult in behalf of some because of the collegiate consignment of their courses coupled with the other tasks that they suffer with to perform. A lot of students feel that they can optimize their prematurely, energy and abilities if they look for help from mask sources to assistants them with their load. Commonly, the said requirements are captivated separately from their terminal exams. Thus, students who make over to people who can further them can service perquisites too by giving them more unceasingly a once to converge on their end exams; allowing those who longing remedy them destruction the other requirements through despite the course. Some assignments which can extras greatly from source backing include research composition, scribble literary works dissertations, essays, and words reports, and theoretical at liberty to moniker a few. Students, however, must be careful in choosing who to pivot to when they need help fulfilling their hypothetical requirements. People or companies who offer support to students who need succour in their scholastic shape are a certain extent common. This may be partly because of the costly call for in favour of their services, which is also the case why of people and companies who offers dishonourable services thrive. Students be required to then work out immense care in choosing who to reorganize to. Deficiency to determine competent and credible people and companies who can assistant them with their hypothetical requirements can development to more evil than good. Coursework Writing may be turned to if students believe the essential on the side of it. Students essential be entirely aware in choosing who to certitude though. Although it is kind to attract safe friends and school mates to refer exceptional people or companies who can be of help, they must also remember that what works fabulously in the service of whole may not incontrovertibly work as splendidly quest of him/her. The proper credentials of those who bid their alleviate necessity be reviewed to impel firm that peerless arrogate is offered. Free Online Dating at online personals site Free Online Dating Sites for singles, with personals, and Matchmaking.

Australian Law Essay Example | Topics and Well Written Essays - 750 words

Australian Law - Essay Example â€Å"Decisions of the High Court are binding on all other courts throughout Australia† (High Court of Australia 2010: The High Court of Australia is the uppermost Court in the judicial system of Australia, the Court of "last resort", in the judicial system of final appeal. It has its origins in the Australian Constitution, Section 71 of which affirms: â€Å"The judicial power of the Commonwealth shall be vested in a Federal Supreme Court, to be called the High Court of Australia, and in such other federal courts as Parliament creates, and in such other courts as it invests with federal jurisdiction† (The High Court of Australia n.d: 1). The High Court shall comprise a Chief Justice and also a lot of other Judges, not below two, as the Parliament has laid down. The fundamental functions of the High Court are to construe and support the Constitution, to construe Federal law and to attend to cases referred from other Courts. Therefore, it is the purpose of this essay to d iscuss why a decision of the High Court on the constitutionality of a Statute will be seen by the Government as a further serious setback to its legislative reform plan than a judgment by a Judge of a State Supreme Court in understanding the meaning of an important provision in the statute, in a way contrary to the Government’s goal.... of this essay to discuss why a decision of the High Court on the constitutionality of a Statute will be seen by the Government as a further serious setback to its legislative reform plan than a judgment by a Judge of a State Supreme Court in understanding the meaning of an important provision in the statute, in a way contrary to the Government’s goal. The Australian Constitution states that the power to create laws is vested in the Parliament. At the same time, the power to understand laws and to judge whether they are relevant in individual cases is vested in the High Court and other Central Courts. Actually, one of the important functions of the High Court is to interpret the Constitution. For instance, the Australian High Court can rule a law to be illegal which is beyond the authority of Parliament to enact and so of no effect. Such a condition would be seen by the Government as an obstruction. The Australian Constitution founds the Federal Government by providing for the Parliament, the Judiciary and the Executive, that is identified as the three pillars of governance or as the policy of â€Å"separation of powers† (Clark 2009: 972). Parliamentary Government means that the Executive Government comes from in the Parliament. Accountable Government means that the Executive Government is accountable to the Parliament. The rule of â€Å"separation of powers† is to prevent an oppressive government. The â€Å"three branches of government† constituted by the Legislature, Judiciary and Executive, work as checks and balances on each other (About Parliament. n.d:1). The Judiciary is â€Å"independent† of the other two arms of Government (1). That independence is one of the main critical safeguards of the democratic system of the country. The Executive is the managerial part of

Understanding business operations Essay Example | Topics and Well Written Essays - 2000 words - 1

Understanding business operations - Essay Example Supply chain management integrates the key business processes, from end user through original suppliers. Companies and corporations involve themselves in a supply chain by exchanging information regarding market fluctuations and production capabilities. Technology can be used effectively to enhance the performance of the supply chain to ensure value propositions at all points of the supply chain. Ensuring superior value propositions based on customer service leads to competitive advantage. If all relevant information is accessible to any relevant company, every company in the supply chain has the possibility to and can seek to help optimizing the entire supply chain rather than sub optimize based on a local interest. This will lead to better planned overall production and distribution which can cut costs and give a more attractive final product leading to better sales and better overall results for the companies involved. The primary objective of a company’s supply chain management is to fulfil customer demands through the most efficient use of resources, including distribution capacity, inventory and labour. A supply chain seeks to match demand with supply and do so with the minimal inventory. Various aspects of optimizing the supply chain include liaising with suppliers to eliminate bottlenecks; sourcing strategically to strike a balance between lowest material cost and transportation, implementing JIT (Just In Time) techniques to optimize manufacturing flow; maintaining the right mix and location of factories and warehouses to serve customer markets, and using location/allocation, vehicle routing analysis, dynamic programming and traditional logistics optimization to maximize the efficiency of the distribution side. Incorporating SCM successfully leads to a new kind of competition on the global market where competition is no longer of the company versus company form but rather takes on a supply chain versus supply chain form. When a firm sustains

The literature relating to Effects of Internet Essay Example for Free

The literature relating to Effects of Internet Essay This chapter discusses the literature relating to Effects of Internet use and Internet Addiction (IA). The first section introduces the background of the Internet and identifies the prevalence of IA. The second section discusses the definitions of addiction and their relevance to IA. Background of the Internet The Internet was established in the early 1960s and subsequently became a mainstream communication vehicle (Moschovitis, Polle, Schuyler, Senft, 1999; Schneider, et al., 2006). Since that time, there has been remarkable growth in the Internet‟s functionality, capacity, accessibility and convenience. These improvements have encouraged more people to use it more often, and it has become a powerful application in modern society. As of 2010, 28.7% of the worlds population used Internet services (Internet World Stats, 2010b). The Internet is a massive, computer-linked network system used globally to access and convey information, either by personal or business computer users; it is also used for communication, research, entertainment, education and business transactions (Kraut, et al., 1998; Schneider, et al., 2006). Today, the Internet can link all online computers so that people can use it to communicate throughout the world (Schneider, et al., 2006). Prevalence of IA. The prevalence of IA has been examined in many countries among school student cohorts (see Table 2.1). IA has been reported at a wide range of rates, from a low of 1% in Greece (Tsitsika, et al., 2009) to a high of 36.7% in Italy (Milani, et al., 2009). Most research has reported a prevalence rate 10% or less, for example, 1.6% in South Korea (Kima et al., 2006), 2% in Norway (Johansson Gà ¶testam, 2004), 2.4% in China (Cao Su, 2006), 4% in South Korea (Lee, et al., 2007), 4.6% in Australia (Thomas Martin, 2010), 6% in Poland (Zboralski, et al., 2009), and 7.1% in China (Lang, Jia, Li, Su, 2008). However, a few studies have reported a high prevalence rate of IA among students, for example, 10.7% in South Korea  (Park, Kim, Cho, 2008), 10.8% in China (Lam, et al., 2009), 18.2% in Taiwan (Ko, et al., 2007), and 36.7% in Italy (Milani, et al., 2009). Internet addiction. The first study of IA was conducted by Young (1996), who reported that 79.88% of 496 general Internet users were classified as Internet dependents, using the 24 Diagnostic Questionnaire DQ via email and telephone survey. IA has increasingly been recognized as a potential problem since the introduction of the term by Goldberg in 1996 (Marshall, 1999). While different approaches to different addictions fill the literature, essentially the same ideas about addiction and many of the same behaviours are being described, whether it is substance dependence, pathological gambling, or technology addiction, (Horvath, 2004; McIlwraith, et al., 1991). IA has generally been defined as an inability to control the use of the Internet, causing psychological, social, family, school and work impairment (Davis, 2001; Young Rogers, 1998). However, the terminology or labels for IA are inconsistent in the literature. This study uses the term IA to encompass all the various terms used in the literature. As yet, there are no standard diagnostic criteria for IA agreed upon in the literature. Nevertheless, most researchers acknowledge the existence of IA. As Griffiths (1998) noted, â€Å"Excessive use of the Internet may not be problematic in most cases but the limited case study evidence suggests that for some individuals, excessive Internet use is a real addiction and of genuine concern† (p. 73). Researchers have also tried to develop an accurate assessment tool in order to diagnose IA. For example, a well known assessment tool to classify IA was introduced by Young (1996) in the form of an eight-item Diagnostic Questionnaire (DQ) which was based on pathological gambling criteria. The DQ utilizes a set of yes/no questions regarding preoccupation with the Internet, the amount of time spent on the Internet, and the negative impacts of the Internet use. Since the introduction of Young’s instrument (Young, 1996), several other assessment tools have been developed.

Symptoms And Treatments In Cystic Fibrosis

Symptoms And Treatments In Cystic Fibrosis Abstract Cystic fibrosis (CF) is one of the most common genetically inherited diseases which can cause premature death in western populations, with 1 in 2000-3000 new born babies being found to be affected by Cystic fibrosis in Europe [1]. The disease is caused by defective chloride ion channels along the epithelial membrane of the lungs, pancreas and other organs; although there are several hypotheses as to how this dysfunction specifically gives rise to the typical symptoms. The complications associated with the disease are varied, the most significant being the build up of abnormally thick excess mucus which can cause impaired function of the lungs and other major organs. Fortunately research into new treatments has significantly improved the life expectancy of people suffering from this disease. This essay discusses the causes Introduction The name cystic fibrosis refers to the generation of cysts in the pancreas and the formation of excess fibrous connective tissue in the lungs. The internal organs which suffer the most damage as a result of this disease are the lungs and the pancreas; although a variety of other organs are also affected. The first clinical recognition of cystic fibrosis didnt occur until the 1930s when its symptoms were observed and characterised by Dr. Dorothy Anderson. The recessive nature of the disease was confirmed in the mid-forties after an investigation involving over one hundred families; although the defective gene that causes the disease wasnt isolated for another forty years when it was discovered in 1989 by reverse genetics. After the breakthrough in the forties general understanding of the disease increased steadily over the next couple of decades with a major clinical advancements in diagnostics occurring in the fifties with the development of the sweat test. As cystic fibrosis is the result of an autosomal recessive disorder, the sufferer will have to of inherited two copies of the mutated gene (one from each parent) in order to be affected by the disease. The mutation takes place in a single gene on chromosome 7. This faulty gene leads to the development of a defective cystic fibrosis transmembrane conductance regulator (CFTR) protein. In healthy people the CFTR proteins form ion channels to transport chlorine ions across the epithelial membrane of the lungs, pancreas, sweat glands and other organs. It is also thought to regulate the activity of other chlorine-selective channels and some cation-selective (sodium ion) channels. Ions can then pass through these channels thereby maintaining the water potential of the cells. When the fine balance of ion concentration is affected less water is able to pass across the epithelial membrane by osmosis causing excess and highly viscous mucus to build up in the affected organs, resulting in severe long-term respiratory and digestive problems. The human lungs are adapted for use in aerobic respiration by providing a thin, moist surface for gas exchange to take place between the pulmonary arteries and the external environment. For gas exchange to be effective the respiratory surface must comply with Ficks law which requires that the surface area is large, moist and thin to enhance permeability. The resulting fibrosis caused by the disease greatly affects the permeability of the lungs and hence reduces their capacity for gas exchange. Molecular mechanisms There are over 1500 types of mutation which can cause a defect in the CFTR protein, the most common of which is a deletion of phenylalanine at position 508 (à ¢Ã‹â€ Ã¢â‚¬  F508) which Is the cause of approximately two-thirds of CF cases. The mutations are categorised into six classes determined by their impact on the resulting functionality of the CFTR channels, ranging from reduced to complete non-function. Class I, II and III mutations all result in the absence or substantial reduction of functional CFTR. Class I mutations cause a complete lack of protein production due to premature stop codons arising in the genetic code whereas class II mutations produce a protein that doesnt fold properly and so is consequently degraded by the cell. In a class III mutation the lack of effective binding with ATP molecules leads to the defective regulation of CFTR and so again is classified as being non-functional. Classes IV and V still permit the development of functional CFTR albeit with reduced capacity for chloride ion transport or with reduced production of functional CFTR in general due to promoter mutations that decrease transcription [2]. Class VI mutations also produce functional CFTR although its degradation is greatly accelerated. The F508 deletion results in a class II mutation. There are four main hypotheses as to how this defective gene causes disease although it is not known whether the disease is caused by one or a combination these hypotheses. Two of these, the low volume and high salt hypotheses, provide a detailed description of the complications that arise as a result of faulty CFTR by taking into account the composition of airway surface liquid (ASL). Low volume hypothesis In the case of the low volume hypothesis it was postulated that there is little to no difference in the salt concentration of ASL between healthy people and those suffering from cystic fibrosis. This hypothesis suggests that the symptoms of cystic fibrosis are caused by a dysfunction of the CFTR gene resulting in damaged or ineffective sodium ion channels. The damage caused is ergogenic and reduces the inhibition of the ion channels leading to the excessive movement of sodium ions from the ASL into the airways. The increased concentration of positively charged sodium ions in the airways then drives the absorption of chlorine ions and water, reducing the volume of ASL and dehydrating mucus. The dehydrated mucus becomes highly viscous and the cilia present on epithelial cells which are used to aid the clearance of mucus and to increase lung surface area become compressed by the mucosal build up. This compression of cilia inhibits the clearance of mucus which then continues to build up, further reducing the lung surface area. The excess mucus can also form hypoxic niches that can harbour colonies of pseudomonas aeruginosa. Build up of mucus physically reduces the lung surface area affecting the efficiency of gas exchange. The mucus build up also increases the compression of cilia on epithelial cells which inhibits clearance by cilia and coughing. High salt hypothesis The high salt hypothesis assumes that the airway surface liquid of healthy individuals has a relatively low salt concentration when compared to the ASL of cystic fibrosis sufferers. It suggests that the symptoms of the disease are caused by the disruption or complete absence of CFTR function which causes excess sodium and chloride ions to be retained in the ASL. This increased retention of chloride ions leads to the ASL having an abnormally negatively charged composition. This abnormality impairs the activity of the bodys natural bactericidal enzymes such as lysozyme which rely on electrostatic interactions to attach to the bacterial cell walls; thus allowing bacterial infection to persist in the hypoxic niches formed within the lungs. Abnormally high inflammation It has been speculated that the defective CFTR itself may be the cause of excessive inflammation in the airways. However there is limited evidence to suggest that the defective CFTR is a cause of excessive inflammation in itself but rather that it interferes with the regulation of autophagy. Autophagy is the process by which defective proteins are degraded in order to maintain the balance between the recycling and synthesis of cellular products, for example the degradation of defective CFTR by the cells own lysosomes. Research indicates that large amounts of defective CFTR inhibits autophagy, leading to an accumulation of aggresomes which can cause inflammation in the lungs [3]. The resulting inflammation is what gives rise to the characteristic scarring of lung tissue. CFTR bind with P. Aeruginosa Chronic bacterial infection is common amongst all cystic fibrosis sufferers, specifically the bacterial species pseudomonas aeruginosa which binds readily to the CFTR protein. In healthy people the body initiates an immune response in order to fight off the infection. In cystic fibrosis suffers there is enhanced binding between p. Aeruginosa and the CFTR protein, the bacterium is also able to bind without initiating an immune response. The compromised immune response combined with reduced ability to clear mucus due to compressed cilia further increases the risk of severe infection. Symptoms Visible characteristics typical amongst suffers include a slightly meagre appearance due to inefficient absorption of nutrients and the famously salty sweat used to confirm CF diagnosis. Low levels of oxygen in the tissues due to impaired gas exchange between the lungs and the bloodstream can cause clubbing of the fingers and toes Salty sweat The salty sweat associated with the disease like so many of its symptoms is again caused by faulty CFTR present on the sweat ducts. As sodium ions leave the sweat ducts through ion channels chloride ions follow through them through the CFTR protein channels. However, in cystic fibrosis patients dysfunctional CFTR channels prevent the outward flow of chloride ions from the sweat ducts. The resulting high chloride ion concentration in sweat ducts creates an electrochemical gradient which pulls more positively charged sodium ions into the ducts where the ions combine to form salt (NaCl). The salt is then secreted through pores in the skin resulting in very salty sweat as very little NaCl is reabsorbed. Salt sweat concentration of greater than 60mEq/L is generally considered significant enough to make a diagnosis, although further test may be required. Although poor growth can pose its own health risks the most severe symptoms are caused by the diseases capacity to cause damage to the internal organs. Endocrine CF is commonly referred to as an exocrine disorder meaning the resulting dysfunction affects glands which secrete their products through a duct to the surface of the body or of an organ, sweat glands and pancreatic ducts being an example of this. However some complications can arise in the bodys endocrine glands, glands which secrete their product directly into the bloodstream. Disorders of the endocrine glands tend to affect the secretion of hormones. Damage to the islets of langerhans within the pancreas can impair the secretion of insulin which can eventually lead to CF related diabetes. Pulmonary Lungs are the predominant source of infection, vulnerable to different species of bacteria although P. Aeruginosa becomes predominant; eventually these bacterial colonies form a biofilm which is difficult to remove with antibiotic treatments. The thickening of mucus creates environmental niches suitable for harbouring bacteria. High levels of infection result in an inflammatory response which often leads to extensive tissue damage and scarring regarded as the characteristic fibrosis of the lungs. The resulting fibrosis damages the epithelium of the lungs, making gas-exchange inefficient. Thick mucus also physically reduces the surface area Implications for other organs The lungs arent the only organs that suffer damage as a result of cystic fibrosis Diverse range of other organs affected, majority of these form a part of the alimentary system Lacking digestive enzymes in the intestines Absence of these digestive enzymes impair patients ability to breakdown and therefore digest their food. This generally results in poor growth but in extreme cases can cause severe malnutrition. Alimentary system all digestive systems The formation of cysts blocks ducts in the liver and pancreas preventing the secretion of essential digestive enzymes and hormones. Blocked ducts prevent secretion of enzymes/hormones? Mainly the pancreas which affects absorption of nutrients and can lead to poor growth in suffers Blockage of ducts in the liver New born babies can suffer from meconium ileus, an inability to pass their first faeces (meconium). The resulting ileus can cause blockages in the intestines that can cause rectal prolapse due to the strain involved in producing a bowel movement. Common associations between cases of meconium ileus and CF led to it being used as a postnatal diagnostic technique. Excess viscous mucus isnt only a problem in the lungs. Organs of the alimentary system can also be affected. Thick mucus can block pancreatic ducts preventing the secretion of vital digestive enzymes into the duodenum. The body is then unable to effectively extract nutrients from the ingested foods. Malabsorption is a common symptom of CF generally resulting in poor growth but in extreme cases can cause severe malnutrition. Fertility problems Fertility problems related to CF usually occur before birth whilst the foetus is still developing. Blocking of or complete absence of the connective tube (vas deferens) between the testes and ejaculatory ducts in males means that although they are not sterile they are unable to conceive children by traditional intercourse. In women thick mucus can cause blockages in the cervix or their ovulation cycle may be disrupted malnutrition as a result of CF related enzyme deficiencies. More than 95% of males with CF are infertile. Percentage of CF infertile source world health organisation Treatments Unfortunately there is currently no cure for CF however there are several treatments that can alleviate some of the symptoms associated with the disease, such as the use of hypertonic saline and enzyme replacement. Treatments such as gene therapy are more geared towards creating a permanent cure for CF, although at this moment in time the technology has not been perfected. Pharmacological treatments Fortunately the CFTRs are not the only chloride ion channels available on lung surface epithelium. Certain drugs can stimulate these other channels. Rcjournal. Stimulate the release of calcium or inhibit sodium channels to offset negative effects of whatever hypothesis. Hypertonic saline Major complications of CF stem from the imbalance in ion concentrations caused by the faulty CFTR gene. From this knowledge a line of treatments were developed in order to restore the ionic imbalance and hence improve the bodys ability to clear thick mucus from the lungs. The answer would need to be a sterile solution; high in salts that could be inhaled to replace the ions which werent being transported across the CFTR channels. The solution, hyper tonic saline, is a cheap and effective treatment for reducing the viscosity of mucus in the lungs. After it is inhaled the solution works by creating an osmotic gradient, drawing water into the airways, rehydrating the mucus causing blockages and reducing its viscosity hence making it easier to cleared and coughed up. Enzyme replacement Enzymes can be *injected* to restore the deficiency created by blockages of pancreatic ducts. Patients undergoing enzyme replacement therapy can expect to see improvements in growth, weight gain and general health as many illnesses arise from poor absorption of nutrients. Nutritional supplements can also be taken to replace those not being absorbed normally. Important short term treatments are giving nutritional supplements to sufferers to relieve malnourishment and promote healthy growth Nutritional plans generally involve high calorie diets rich in vitamins such as vitamin D to develop strong bones and prevent osteoporosis. Gene therapy Soon after the discovery of the defective gene in 1989 efforts were invested in finding a therapy that could target the disease at its genetic roots. Discovery of an effective method of gene therapy would open a virtual goldmine in treating not only cystic fibrosis but also other genetic diseases. One of the current gene therapy techniques for the treatment of CF involves the use of adenoviruses carrying vectors containing corrected copies of the CFTR gene. The adenoviruses carry double stranded DNA which is deposited in the nucleus of the host cell and then transcribed in the same way as the host cells own DNA. However, as this is an example of somatic gene therapy, the DNA of the adenovirus wont integrate with the host genome and the gene will not continue to be expressed after cellular division. This means the effects are not permanent and patients will require subsequent treatments to maintain the effect. There are of course risks associated with the use of viruses to incorporate functional DNA. Even though the viruses being used are non-pathogenic the presence of a foreign body can still initiate an immune response; the resulting inflammation can be dangerous for patients who are already at high risk of excessive inflammation due to immunocomprimisation. An alternative to adenoviruses are adeno-associated viruses (AAV). AAV vectors are non-pathogenic and have been shown to have a lower prevalence to neutralising antibodies when com pared to adenoviruses in vitro [4]. However they do have a relatively small genome of ~4.8 kilobases, with most gene treatments requiring the complete replacement of the viral genome. As a result of this, research is being targeted towards more effective means of gene therapy with tests being carried out with AAV and lipid-vectors. There is a trade off between the effectiveness of the method used to induce the vector. Viral vectors are more effective at integrating the vector into the host DNA compared to lipid vectors, but there is the increased risk of an immune response. Lung transplant In the most severe cases where patients are suffering from chronic infection lung transplants can be carried out where appropriate. In these cases both lungs need to be transplanted in order to prevent the new lung from being contaminated by existing bacterial populations currently present in the patient. Antibiotics Antibiotics tend to be used prophylacticaly as a pre-emptive measure for preventing infection. One common type of drugs used in treatments are macrolide antibiotics can suppress obstructive secretions in airways The macrolide antibiotics work by binding to the 50s subunit of the bacterial ribosome, thereby inhibiting protein synthesis. Conclusion The overall outlook for patients with CF has improved dramatically over the past eighty years since the first clinical recognition in the 1930s. A greater understanding of genetics has enhanced both the fields of clinical diagnostics and treatments. The future of treatments points towards gene therapy, we currently have the technology to do this but further research is needed to overcome the major obstacles such as more efficient transfer and getting the gene expression to last longer. [*http://www.who.int/genomics/public/geneticdiseases/en/index2.html#CF] http://www.medscape.com/viewarticle/576200_2 http://www.nature.com/ncb/journal/v12/n9/full/ncb2090.html http://www.nature.com/gt/journal/v6/n9/full/3300994a.html Figure 1. Sweat chloride concentrations related to cystic fibrosis (CF) diagnosis. Revised and reprinted by permission from Davis PB. Cystic fibrosis. Pediatr Rev 2001;22:257-264.Figure 1